Journal: Materials Today Bio

Article Title: Biomimetic cancer cell membrane-coated liposomal nanocarriers loaded with silibinin suppress gastric cancer progression via SNHG1/miR-383-5p/HSP90AA1 axis-mediated PI3K/AKT pathway inhibition

doi: 10.1016/j.mtbio.2025.102744

Figure Lengend Snippet: Construction and characterization of biomimetic nanovesicles CLip@Sil loaded with silibinin and modified with GC cell membranes. Note: (A) Schematic diagram illustrating the synthesis process of CLip@Sil, including extraction of HGC-27 cell membrane proteins, liposome preparation, drug loading, and final assembly, created in BioRender; (B) SDS-PAGE and Western blot analysis of Pan-cadherin, COXIV, and Histone H3 expression in different groups to assess membrane protein purity; (C) IF co-localization showing the overlap between DiR-labeled cell membrane and C6-labeled Lip@Sil signals in CLip@Sil, bar: 50 μm; (D) Particle size distribution of Lip@Sil and CLip@Sil determined by DLS; (E) Zeta potential measurement of both nanovesicle types to assess surface charge characteristics; (F) TEM images of extracted cell membrane, Lip@Sil, and CLip@Sil showing that CLip@Sil exhibits a typical core–shell structure, bar: 100 nm; (G) HPLC analysis of silibinin encapsulation efficiency in Lip@Sil and CLip@Sil; (H) Dialysis-based release study showing silibinin release rates from CLip@Sil under pH 5.0 and pH 7.4 conditions over 24 h. All experiments were performed in triplicate.

Article Snippet: After BCA quantification, 40 μg of protein per sample was loaded onto 10 % SDS-PAGE gels (Cat. No. 4561033, Bio-Rad, USA), followed by membrane transfer.

Techniques: Modification, Extraction, Membrane, SDS Page, Western Blot, Expressing, Labeling, Zeta Potential Analyzer, Encapsulation